Description:

Plasmids-

• pDD82- minimal shuttle vector between Escherichia coli and Rhodococcus opacus PD630 that contains the pB264 origin of replication.
• pDD112- a shuttle vector between Escherichia coli and Rhodococcus opacus PD630 that contains the pNG2 origin of replication and a codon optimized chloramphenicol resistance marker.
• pDD120 is a plasmid construct containing the bacteriophage derived recombinases Che9c60 and Che9c61 expressed by a constitutive promoter on the pB264 vector backbone.

Publication:

DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS. Molecular Toolkit for Gene Expression Control and Genome Modification in Rhodococcus opacus PD630. ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 10.1021/acssynbio.7b00416. Epub 2018 Feb 5.