mAb 4E3 was derived by immunization of β₂m-deficient BALB/c mice with a keyhole limpet hemocyanin-conjugated peptide corresponding to mMR1 residues 130–153 (L A M D Y V A H I T K Q A W E A N L H E L Q Y Q). Hybridomas were produced by standard methods and screened by anti-peptide ELISA.

Publication: Biochemical Features of the MHC-Related Protein 1 Consistent with an Immunological Function