— Stable zebrafish line that shows calcium signaling within the pancreatic beta cells. The line was generated using the calcium sensing fluorophore gCAMP and integrated using the TOL2 transcription factor. Publication: Beta-cell excitability and excitability-driven diabetes in adult Zebrafish islets
— Dr. Dougherty developed a collection of mouse lines with deletions of Gft2i and Gtfird1 in various combinations. The genes are related to Williams Beuren locus. Publications:
— Collagen antibodies that bind to the major collagen IV component of the kidney glomerular basement membrance. The antbiodies reaction with mouse, rat, and human chains and were derived using the noncollagenous NCI domains at the COOH-termini in rabbit antiserum to mouse. Collagen IV alpha3 chain: 353 Collagen IV alpha4 chain: 356 Collagen IV alpha5 chain: 358
— Mouse line carrying a mutation in Col4a3 backcrossed to 129S1/Sv1mJ strain. The mice show kidney failure at 10-12 weeks of age to enable rapid drug screening. Publication: A mouse collagen4α4 mutation causing Alport glomerulosclerosis with abnormal collagen α3α4α5(IV) trime…
— Technology Description An interdisciplinary team at Washington University has developed low-cost, easy-to-use, nanoparticles that can preserve antibodies, proteins and other biological molecules without refrigeration. This technology employs metal organic frameworks (MOF) that can be used in two w…
— Chemically inducible PiggyBac transposase constructs. The plasmids that contain mutated destabilized domains, FKBP and DHFR, were purchased from Addgene with ID 31763 and 29326, respectively. The PBase-ERT2 plasmid (mPBase-L3-ERT2) was a kind gift from Dr. Allen Bradley . The coding sequence of mPBa…
— Plasmids for expression of phenyl alanine tRNA synthase from Toxoplasma gondii, Plasmodium falciparum and Homo sapiens. These include wild type and various mutant forms.
— Transgenic mouse strain designed and developed by Dr. Conrad Weihl with a mutation of the DNAJB6 gene at the F90I protein. This homologous with the human form of muscular dystrophy with patients having a genetic variant at the F89I protein.
— Influenza virus containing a GFP reporter gene in one of the gene-segments. It allows for high throughput screening of compounds or host factors required for influenza virus infection and replication.
— Six recombinant human monoclonal antibodies that are directed against the conserved stalk region of influenza virus HA. This stalk region is currently being assessed as a potential candidate as a universal influenza vaccine. These antibodies can be used as diagnostics and also as validation tools fo…
— Plasmids harboring 3HB with different split intein: pWB076-pE8a-GpC10-3HBt-CfaN10; pWB088-pE8a- CfaC10-3HBt-NrdJN10; pWB079-pE8a-NrdJC10-3HBt-GpN10. Enabling a new process to compare nanostructures.
— This is a transgenic mouse line that expresses the Diphtheria toxin receptor in the myocardium of the mouse heart. Upon administration of Diphtheria toxin cardiomyocytes undergo rapid cell death and mice develop heart failure. This mouse line provides a simple a noninvasive method to model cardiac i…
— Two plasmids each containing a single murine mutant tumor neoantigen: a) retroviral MSCV-IRES encoding full length mutant lamanin alpha 4 (mLama4) b) retroviral MSCV plasmid encoding full length mutant integrin beta 1 (mItgb1)
— A plasmid containing the E. colil ColE1 origin of replication, a chloramphenicol (antibiotic) resistance cassette, and a tetracycline-inducible antisense RNA gene regulator containing a target binding region (TBR) and the synthetic MicF M7.4 Hfq binding site for additional stability in the RNA molec…
— BOOM TCRtg mice express a T cell receptor specific for the Bt4295(541-554) epitope of the commensal bacteria Bacteroids thetaiodaomicron. This is the first mouse TCRtg line developed specific for B. theta.
— New combinations of labeled membrane proteins and G-protein subunits. These include twocolor HRas and single color CD86 controls as well as labels beta and gamma subunits of the heterotrimeric G-protein complex. All of these have been built using plasmids from Addgene.
— These plasmids contain mammalian cell expression promoters and can be used for AAV production. Some plasmids encode a calcium reporter (GCaMP) and photoactivatable fluorescent reporter (PAmCherry). Others plasmids encode a calcium reporter (GCaMP) and one of the vomeronasal receptor genes; two gen…
