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Carbon aerosol gel from continuous negative gravity reactor
Liu, Pai ; Chakrabarty, Rajan
T-017977

— A team of engineers from Washington University has developed a unique aerosol gel made from volume-spanning, semirigid networks of organic nanoparticles. Although the ultralow density and high surface area of these types of aerogels make them extremely useful for air and water purification, their pr…

Monoclonal Antibodies Against Zika Virus and Purified Zika E Protein
Nelson, Christopher ; Platt, Derek ; Zhao, Haiyan ; Fremont, Daved ; Fernandez, Estefania ; Diamond, Michael
T-016168

— To generate a panel of antibodies against ZIKV, we serially infected Irf3/ mice 30 days apart with ZIKV MR-766 (Uganda, 1947) and ZIKV H/PF/2013 (French Polynesia, 2013). Irf3/ mice were used instead of wild-type (WT) mice, because ZIKV strains are deficient in evading type I interferon-mediated imm…

Learnable, scalable, energy efficient analog-to-digital interfaces and their automated design
Chakrabarti, Ayan ; Zhang, Xuan "Silvia"
T-017967

— Technology Description Engineers in Prof. Xuan “Silvia” Zhang’s laboratory have developed a unifying design and optimization paradigm to automate the design of analog-to-digital interfaces and create scalable, general purpose analog and mixed signal (AMS) blocks that employ machi…

TZ4877 and 18F-radiolabeling precursor TZ50153
Tu, Zhude "Will"
T-017602

— TZ4877 is a ligand for the sphingosine-1-phosphate (S1P) receptor 1 in the CNS, developed and radiolabeled with fluorine-18. TZ4877 is the standard (non-radioactive compound) which is used for HPLC analysis of the S1P1 PET tracer, [18F]TZ4877. TZ50153 is the radiolabeling precursor of the PET tracer…

Mpc2 floxed mice
Finck, Brian
T-018821

— Using embryonic stem cells from the European knockout mouse consortium (EUCOMM), researchers developed transgenic mice with loxP sites flanking exon 3 of the mitochondrial pyruvate carrier 2 (Mpc2) gene. MPC2 is a component of the MPC complex, which is involved in the transport of pyruvate across th…

LDLR luciferase reporter in U87 cells
Holtzman, David
T-017684

— U87 cells that express guassia luciferase under the control of the human LDLR promoter with constitutive HRP expression. WU878: PhLDLR/guassia luciferase WU988: pTL/guassia luciferase

IRF8 +32 KO mice
Murphy, Kenneth
T-018830

— Researchers first identified and then used CRISPR to delete an enhancer located at +32 kb of the Irf8 transcriptional start site in mice. Knocking out this enhancer revealed that interferon regulatory factor 8 (IRF8) is required for the development of classical type 1 dendritic cells (cDC1s) but not…

E. coli plasmids and strains for biofuel synthesis
Xiao, Yi ; Jiang, Wen ; Zhang, Fuzhong
T-017688

— pA5c-tesA, pA8c-tesA-mleuABCD, pE2k-alsS-ilvCD Researchers used a standard restriction digestion cloning based on the Biobrick platform or Gibson assembly to construct the plasmids. E. coli DH10B was used for cloning. These plasmids were designed to increase branch chain fatty acid production. Pub…

Nf1 Optic Glioma Stem Cells
Gutmann, David
T-017720

— Using a series of Nf1 genetically-engineered mouse strains, researchers isolated low-grade glioma stem cells (CD133+ cells) for in vitro and in vivo brain tumor studies. These lines are authenticated optic glioma stem cells, with the ability to self-renew at limiting dilutions; express stem cell mar…

ABCA3-GFP expression constructs
Wambach, Jennifer ; Cole, F. Sessions
T-017958

— This technology includes a plasmid with the full length ATP-binding cassette transporter A3 (ABCA3) cDNA cloned into the pAcGFP1-N1 vector and another plasmid with the ABCA3-GFP cDNA inserted into the pShuttle vector. Mutations in ABCA3 lead to severe neonatal respiratory distress syndrome and child…

Six2-GFP reporter iPSC line (subject to GE – GFP license)
Humphreys, Benjamin
T-017760

— Researchers used CRISPR/Cas9-mediated gene targeting to knock in a GFP-P2A cassette into the ATG of the Six2 gene in human BJFF.6 induced pluripotent stem cells. The cells exhibit GFP expression in Six2+ cells when induced to differentiate towards the renal lineage. Six2 expression marks a progenito…

Mutant Zebrafish with chemically induced point mutations
Solnica-Krezel, Lilianna
T-017763

— Mutant zebrafish animals harboring chemically induced point mutations in the genome. Animals: spon1b fh440, kiss1 stl82, kiss1rb fh438, pon2 fh450

Automated, Improved Deformable Image Registration for Radiation Therapy and other Clinical Applications
Yang, Deshan
T-017324

— Technology Description Prof. Deshan Yang and colleagues have developed automated systems and software toolkits to provide more accurate deformable image registration (DIR) for adaptive radiotherapy and other clinical applications. In particular, they have automated tedious and labor-intensive DIR …

Chimeric Artificial Interference RNA (aiRNA) for RNAi-based cancer therapy
Wang, Xiaowei
T-014021

— Technology Description In search of more effective cancer treatments, inventors in Dr. Xiaowei Wang’s lab at Washington University School of Medicine have developed artificial interference RNA (aiRNA). aiRNA is a patented chimeric RNAi that combines the multi-target abilities of miRNAs and t…

Armenian Hamster Anti-Mouse Tapasin Antibody, clone 5D3
Wang, Xiaoli ; Lybarger, Lonnie ; Hansen, Ted
T-016628

— Target: Amino acids 11-34 of mouse tapasin. Application(s): Western Blotting Armenian hamster mAb (5D3) was produced to peptide EDAGGGGLSKKPATLLLRH GPRGP, representing residues 11–34 on mouse Tapasin (TPN) (44). TPN mAb was found to be specific for mouse TPN, as determined in a Western blot analysis of cell lysates of .221 (human TPN-positive) cells, .220 (human TPN-n…

St. Louis isolate of Bourbon virus (BRBV-STL)
Boon, Adrianus "Jacco"
T-018815

— This strain of the Bourbon virus was isolated from the serum of an infected St. Louis-area patient. The isolated virus was used to demonstrate in vitro and in a mouse model that the antiviral drug favipiravir has prophylactic and therapeutic efficacy against the Bourbon virus, indicating it as a goo…

Mouse anti-MR1 hybridomas, clone 4E3 and 6D1
Wang, Xiaoli ; Hansen, Ted
T-017622

— mAb 4E3 was derived by immunization of β2m-deficient BALB/c mice with a keyhole limpet hemocyanin-conjugated peptide corresponding to mMR1 residues 130–153 (L A M D Y V A H I T K Q A W E A N L H E L Q Y Q). Hybridomas were produced by standard methods and screened by anti-peptide ELISA. …

Armenian Hamster Anti-Mouse Properdin Monoclonal Antibody (clone H4)
Bertram, Paula ; Hourcade, Dennis
T-014378

— Target: Full length mouse properdin Application(s): Western blot and immunofluorescenceTwo functional properties of one of the two hamster antibodies have been examined, the H4 subclone of the 3D12 clone. In experiments the H4 antibody was used to identify mouse properdin present in histological sections, to block mouse properdin function in vitro, and to ameliorate complement alterna…

Fibroblast-Derived Cell Line Producing Wnt3a, R-spondin 3, & Noggin
Miyoshi, Hiroyuki ; Stappenbeck, Thaddeus
T-014239

— Tangible Tool: Growth factor over-expressing cell line. Notes: Produces conditioned media to grow various mammalian tissue stem cells.These supportive L-WRN cells can produce conditioned media containing Wnt3a, R-spondin, and Noggin for the the support of gastrointestinal stem cells. This system can be used to make stem cell enriched epithelial spheroids from the mouse gastrointestine. Publication: In vitro expansion and genetic…

New PARP-1 Inhibitors for Imaging PARP-1 Expression in Vivo
Rogers, Buck ; Xu, Jinbin ; Chu, Wenhua ; Zhou, Dong
T-018730

— [18F]WC-DZ-F is a derivative of [18F]FluorThanatrace and a radiotherapeutic agent. [18F]WC-DZ-F has suitable biodistribution profile, favourable logP and super metabolic stability in vivo. We have demonstrated that [18F]WC-DZ-F is capable of imaging PARP-1 expression in PC-3 prostate cancer xenograf…