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Hamster Hybridoma producing Monoclonal Antibody against Mouse CXCR3
Schreiber, Robert
T-005883

— Target: C-X-C chemokine family member (CXCR3). Expressed on NK cells, activated T lymphocytes, select endothelial and epithelial cells.Hamsters were immunized with a multiple antigens peptide-8 (MAPS-8) encompassing amino acids 1 to 37 of mouse CXCR3 in complete Freund’s adjuvant, and boosted with peptide in incomplete Freund’s adjuvant. Hamsters showing ELISA seropositivity for CXCR3 peptide were boosted and hybridomas gen…

Murine Hybridoma producing IgG2b Monoclonal Antibody to Human CD85h (ILT1)
Colonna, Marco
T-005807

— Target: Cluster of Differentiation 85h protein, a.k.a. Ig-Like Transcripts 1. Notes: Clone name is 24.Wistar rats were immunized with ILT1/FLAG-transfected RBL cells. ILT1 mAb was selected by flow cytometry for staining ILT1/FLAG-transfected RBL cells. This antibody can be used to study myeloid cell activation. Publication: Human Myeloid Cells Express an Activating ILT1 Receptor (ILT1) That Ass…

Mouse Hybridoma producing IgG2b Monoclonal Antibodies to Human FcRL4
Wilson, Timothy ; Colonna, Marco
T-007612

— Target: Fc Receptor Like 4 protein. Notes: Clone available is 413D12. Used in flow cytometry, immunoprecipitation, and functional studies.Balb/c mice were immunized three times at two week intervals with paraformaldehyde-fixed FcRL4 stable transfectants, first in CFA, then twice in IFA + CpG 1826. Three days prior to fusion with SP2/0 cells, the final immunization was performed by IP injection of irradiated cells in PBS. Hybridoma clo…

Rat Hybridoma producing IgG2a Monoclonal Antibodies to Murine CD226 (DNAM-1)
Colonna, Marco
T-007610

— Target: Cluster of Differentiation 226 protein, a.k.a. DNAX Accessory Molecule 1. Notes: Clone available is 480.1Inventors generated the antibodies by immunizing rats with RBL cells transfected with mDNAM-1 cDNA (RBL–mDNAM-1) or mouse CD96 cDNA (RBL-mCD96) and selecting hybridomas that reacted with 293–mDNAM-1 or 293-mCD96 transfectants but not with untransfected 293 cells. Publication: DNAM-1 pro…

Rat Hybridoma producing IgG2a Monoclonal Antibodies to Murine CD155 (TAGE4)
Colonna, Marco
T-007611

— Target: Cluster of Differentiation 155 protein, a.k.a. the Polio Virus Receptor. Notes: Clone available is 4.24. Used in flow cytometry.To generate the antibodies, the researchers immunized rats with a recombinant protein consisting of the mCD155 extracellular region fused to human Fc and selected hybridomas that reacted with RMA-S–mCD155 transfectants but not with untransfected RMA-S cells. Publication: DNAM-1 promotes activ…

Hnf4a-Foxa1 fusion plasmid
Morris, Samantha
T-019160

— Plasmid with fused Hnf4a and Foxa1 genes to express as a single transcript. Individual genes were obtained from Addgene.

DNA plasmids to control gene expression in E. coli
Hoynes-O'Connor, Allison ; Moon, Tae Seok
T-019135

— These plasmids contain target binding regions (TBRs) targeting rfp that were placed under the control of pTet via blunt end lifgation. Hfq binding sites that had been identified from the literature were inserted adjacent to the TBR sequence using either blunt end ligation or Golden Gate assembly, de…

Transgenic mouse lines with markers of muscle development
Sanes, Joshua
T-019127

— Myl-CFP Mice The coding sequence for the enhanced CFP was inserted downstream of ~5kb of putative regulatory sequences from the mouse myosin Type I heavy chain gene. Transgenic mice were generated by pronuclear injection using standard methods. JAX Stock No. 06922 MyIIA-RFP Mice A ~100kb genomic…

Lyz2Cre;Talin-1fl/fl mice
Randolph, Gwendalyn
T-019085

— Lyz2Cre;Talin-1fl/fl mouse strain Inventors crossed Talin-1fl/fl mice (received from Oklahoma Medical Research Foundation) with Lyz2Cre mice (available at the Jackson Laboratory) to generate Lyz2Cre;Talin-1fl/fl mice.

Floxed ChAT mouse strain
Burgess, Robert ; Sanes, Joshua
T-019107

— This mouse strain has loxP sites flanking exons 3 and 4 of the ChAT gene. There is a Neo gene in the intron between exons 4 and 5. Mating these mice to A-Cre mice generates a null allele in subsequent generations, in which no ChAT protein or activity is detectable. The mice are maintained in the C57…

Chimeric murine anti-FimH Monoclonal Antibodies
Ellebedy, Ali
T-019117

— These are recombinant chimeric murine monoclonal antibodies that are directed against FimH, an adhesin within uropathogenic E coli (UPEC). The monoclonal antibodies were isolated from mice immunized with adjuvanted FimCH, but are expressed on a human IgG1 backbone and hence they are chimeric.

Small molecule inhibitors to prevent tetracycline resistance
Park, Jooyoung ; Vogel, Joseph ; Wencewicz, Timothy ; Forsberg, Kevin ; Gasparrini, Andrew ; Dantas, Gautam ; Tolia, Niraj
T-016593

— Disease indication – Tetracycline resistant infections Drug format – Small molecule (anhydrotetracycline (aTC) or analogs) Drug class – Combination therapy Research stage and Preliminary data Target – Tetracycline destructases (Tet(X), Tet(50), etc) Background &ndas…

Machine learning method for fast, reliable quality assurance of patient imaging in radiation therapy or computer-aided detection
Wooten, Hasani ; Kavanaugh, James ; Li, Hua ; Mutic, Sasa ; Green, Olga ; Altman, Michael
T-013577

— Technology Description A team of researchers at Washington University has created a machine learning method to quickly and reliably validate patient contours in digital medical images for radiation therapy and computer aided image analysis. Currently, automated contouring tools for delineating tu…

Nanocomposite fertilizer for efficient phosphorus uptake and sustainable agriculture
Raliya, Ramesh ; Biswas, Pratim
T-016378

— Technology Description Engineers in Prof. Pratim Biswas’ laboratory have developed a nanoparticle fertilizer that increases phosphorus uptake and plant yield while reducing environmental damage and strain on natural resources. Increasing global food demand has prompted the large-scale use o…

Murine Hybridoma producing IgG1 Monoclonal Antibody to Human CD96
Colonna, Marco
T-005414

— Target: Cluster of Differentiation 96 protein, a.k.a. T Cell-Activated Increased Late Expression protein. Notes: Clone name is MX92.39.mAbs against CD96 (clone NK92.39, mouse IgG1) was obtained by immunizing mice with NK92 cells. This antibody can be used to study how NK cells recognize tumors. Publication: Cutting Edge: CD96 (Tactile) Promotes NK Cell-Target Cell Adhesion by Interacting with the Poliovirus Receptor (CD155)

Murine Hybridoma producing IgG1 Monoclonal Antibody to Human ILT3
Colonna, Marco
T-005803

— Target: Immunoglobulin transcript 3 (ILT3). Notes: Clone name is ZM4.1.To generate the hyrbidomas, 10-week-old, female BALB/c mice received an initial injection of ILT3 human IgG1 fusion protein mixed with Alu-Gel-S behind the neck. 4 weeks later, they were given a booster immunization with the same immunogen, followed after 2 weeks by a final injection of purified ILT…

A genetic mouse model of human Cantu Syndrome based on Abcc9em1Nich SUR2[A476V] mutation
Remedi, Maria ; Nichols, Colin
T-019072

— This mouse model was generated by using CRISPR/Cas9 to mutate the ABCC9 gene. The mouse model described here could be used as a pre-clinical model for testing Cantu Syndrome therapies. Publication: Cardiovascular consequences of KATP overactivity in Cantu syndrome

A genetic mouse model of human Cantu Syndrome based on Kcnj8em1Nich[V65M] mutation
Remedi, Maria ; Nichols, Colin
T-019073

— This is a CRISPR/Cas9 germline mutation in the KCNJ8 gene, equivalent to a human Cantu Syndrome mutation. The mouse is therefore a genetic model of human Cantu Syndrome. This mouse model can be used for pre-clinical testing of targeted therapy for Cantu Syndrome. Publication: Cardiovascular consequ…

Stat1-flox mouse
Holtzman, Michael
T-019075

— This is a trangenic mouse with the floxed Stat1 gene. This mouse can be breed with whole body or tissue specific Cre lines to knockout out STAT1 – which is a transcription factor that is essential for the interferon signaling pathway. Publication: Respiratory Enterovirus (Like Parainfluenza Virus)…

Murine Hybridoma producing IgG1 Monoclonal Antibody to Human SIRP-B2
Colonna, Marco
T-005412

— Target: Cluster of Differentiation 172g protein, a.k.a. Signal Regulatory Protein Beta-2. Notes: Clone name is LSB2.20.Monoclonal antibodies were raised in a 4-to 8 week-old female Balb/c mouse by immunozation with a recombinant GST fusion protein containing the whole extracellular domain of SIRPB1. These can be used to study T-cell activation and the induction of antigen-specific cytotoxic T-lymphocyte responses by…