— This technology includes a plasmid with the full length ATP-binding cassette transporter A3 (ABCA3) cDNA cloned into the pAcGFP1-N1 vector and another plasmid with the ABCA3-GFP cDNA inserted into the pShuttle vector. Mutations in ABCA3 lead to severe neonatal respiratory distress syndrome and child…
— Researchers used CRISPR/Cas9-mediated gene targeting to knock in a GFP-P2A cassette into the ATG of the Six2 gene in human BJFF.6 induced pluripotent stem cells. The cells exhibit GFP expression in Six2+ cells when induced to differentiate towards the renal lineage. Six2 expression marks a progenito…
— Technology Description Prof. Deshan Yang and colleagues have developed automated systems and software toolkits to provide more accurate deformable image registration (DIR) for adaptive radiotherapy and other clinical applications. In particular, they have automated tedious and labor-intensive DIR …
— Technology Description In search of more effective cancer treatments, inventors in Dr. Xiaowei Wang’s lab at Washington University School of Medicine have developed artificial interference RNA (aiRNA). aiRNA is a patented chimeric RNAi that combines the multi-target abilities of miRNAs and t…
— Target: Amino acids 11-34 of mouse tapasin. Application(s): Western Blotting Armenian hamster mAb (5D3) was produced to peptide EDAGGGGLSKKPATLLLRH GPRGP, representing residues 11–34 on mouse Tapasin (TPN) (44). TPN mAb was found to be specific for mouse TPN, as determined in a Western blot analysis of cell lysates of .221 (human TPN-positive) cells, .220 (human TPN-n…
— This strain of the Bourbon virus was isolated from the serum of an infected St. Louis-area patient. The isolated virus was used to demonstrate in vitro and in a mouse model that the antiviral drug favipiravir has prophylactic and therapeutic efficacy against the Bourbon virus, indicating it as a goo…
— mAb 4E3 was derived by immunization of β2m-deficient BALB/c mice with a keyhole limpet hemocyanin-conjugated peptide corresponding to mMR1 residues 130–153 (L A M D Y V A H I T K Q A W E A N L H E L Q Y Q). Hybridomas were produced by standard methods and screened by anti-peptide ELISA. …
— Target: Full length mouse properdin Application(s): Western blot and immunofluorescenceTwo functional properties of one of the two hamster antibodies have been examined, the H4 subclone of the 3D12 clone. In experiments the H4 antibody was used to identify mouse properdin present in histological sections, to block mouse properdin function in vitro, and to ameliorate complement alterna…
— Tangible Tool: Growth factor over-expressing cell line. Notes: Produces conditioned media to grow various mammalian tissue stem cells.These supportive L-WRN cells can produce conditioned media containing Wnt3a, R-spondin, and Noggin for the the support of gastrointestinal stem cells. This system can be used to make stem cell enriched epithelial spheroids from the mouse gastrointestine. Publication: In vitro expansion and genetic…
Mouse and Human Anti-MR1 Hybridoma Fremont, Daved ; Gilfillan, Susan ; Myers, Nancy ; Cella, Marina ; Colonna, Marco ; Hansen, Ted
T-013681
— Target: Major Histocompatibility Complex, Class 1-Related Protein Notes: Clone available is 26.5Hybridoma clone 26.5 secreting mAb 26.5 to human and mouse MR1, an MHC-like molecule that restricts the activation of anti-microbial MIAT cells. Publication: Evidence for MR1 Antigen Presentation to Mucosal-associated Invariant T Cells
— Disease indication – cancer, particularly lung, breast, colon, and prostate cancer or melanoma (cancers where chemerin is down-regulated at the tumor site) Drug format – engineered fusion protein Drug class – first in class Research stage and Preliminary data Target &ndash…
— Bnip3-KO mice: These were generated by replacing exons 2 and 3 with a neomycin resistance cassette. Recombinations were identified via southern blot. Publication: Inhibition of ischemic cardiomyocyte apoptosis through targeted ablation of Bnip3 restrains postinfarction remodeling in mice Nix KO m…
— o pDD56: a shuttle vector between Escherichia coli and Rhodococcus opacus PD630 that contains arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance. o pDD57: a shuttle vector between Escherichia coli and Rhodococcus opacus PD630 that contains a constitutively expressed GFP+ on…
— Transgenic HoxB5 conditional DTR (cDTR) mice have been generated using CRISPR mediated targeting of the HoxB5 gene (exon2) in fertilized wildtype C57Bl/6 eggs that were then microinjected into pseudopregnant wildtype female mice. The construct targeted under the controls of the endogenous HoxB5 prom…
— Knock-in mice with a mutation in alpha-crystallin (cryaa-R49C) that causes cataracts. A knock-in plasmid was generated by cloning the 5′ and 3′ arms of the mouse RAcrystallin gene, followed by mutation and homologous recombination in mouse embryonic stem cells, which were implanted into …
— This self-replicating plasmid is a derivative of pPMQAK1-BBa_P1010 and contains an expression-enhanced YFP gene under the control of an inducible Trc promoter. This plasmid was used to demonstrate that transgenic DNA could be introduced to Synechococcus UTEX 2973 cyanobacteria cells using conjugatio…
— WUSTL Technology 007646 Technology Description Researchers at Washington University in St. Louis (WUSTL) have developed a novel TRAIL (TNF-related apoptosis inducing ligand)-based anticancer drug platform, designated TR3. TRAIL has potent and specific apoptotic activity against tumor cells. Thus,…
— WUSTL Technology 011885 Technology Description Researchers at Washington University in St. Louis have developed a strategy to specifically target the anticancer therapeutic TR3 to cancer cells using the mesothelin/MUC16 interaction. Mesothelin is a GPI linked cell surface protein expressed in mes…
— WUSTL Technology 016481 Technology Description Researchers at Washington University in St. Louis have developed an additional targeting strategy to provide more flexibility and improve the bioactivity of the anticancer therapeutic TR3. This strategy targets TR3 to mesothelin, a tumor biomarker fr…
