by | Dec 18, 2019 | Diamond, Michael, Fernandez, Estefania, Fremont, Daved, Nelson, Christopher, Platt, Derek, Zhao, Haiyan
— To generate a panel of antibodies against ZIKV, we serially infected Irf3/ mice 30 days apart with ZIKV MR-766 (Uganda, 1947) and ZIKV H/PF/2013 (French Polynesia, 2013). Irf3/ mice were used instead of wild-type (WT) mice, because ZIKV strains are deficient in evading type I interferon-mediated imm…
by | Dec 17, 2019 | Yang, Deshan
— Technology Description
Prof. Deshan Yang and colleagues have developed automated systems and software toolkits to provide more accurate deformable image registration (DIR) for adaptive radiotherapy and other clinical applications. In particular, they have automated tedious and labor-intensive DIR …
by | Dec 16, 2019 | Wang, Xiaowei
— Technology Description
In search of more effective cancer treatments, inventors in Dr. Xiaowei Wang’s lab at Washington University School of Medicine have developed artificial interference RNA (aiRNA). aiRNA is a patented chimeric RNAi that combines the multi-target abilities of miRNAs and t…
by | Dec 12, 2019 | Hansen, Ted, Lybarger, Lonnie, Wang, Xiaoli
— Target: Amino acids 11-34 of mouse tapasin.
Application(s): Western Blotting
Armenian hamster mAb (5D3) was produced to peptide EDAGGGGLSKKPATLLLRH GPRGP, representing residues 11–34 on mouse Tapasin (TPN) (44). TPN mAb was found to be specific for mouse TPN, as determined in a Western blot analysis of cell lysates of .221 (human TPN-positive) cells, .220 (human TPN-n…
by | Dec 11, 2019 | Bertram, Paula, Hourcade, Dennis
— Target: Full length mouse properdin
Application(s): Western blot and immunofluorescence
Two functional properties of one of the two hamster antibodies have been examined, the H4 subclone of the 3D12 clone. In experiments the H4 antibody was used to identify mouse properdin present in histological sections, to block mouse properdin function in vitro, and to ameliorate complement alterna…