Summaries by Inventor Boon, Adrianus

Influenza GFP reporter virus
Boon, Adrianus "Jacco"
T-019202

— Influenza virus containing a GFP reporter gene in one of the gene-segments. It allows for high throughput screening of compounds or host factors required for influenza virus infection and replication.

St. Louis isolate of Bourbon virus (BRBV-STL)
Boon, Adrianus "Jacco"
T-018815

— This strain of the Bourbon virus was isolated from the serum of an infected St. Louis-area patient. The isolated virus was used to demonstrate in vitro and in a mouse model that the antiviral drug favipiravir has prophylactic and therapeutic efficacy against the Bourbon virus, indicating it as a goo…

Plasmid (pHW2000): Development of a reverse genetics system for Bourbon virus
Boon, Adrianus "Jacco"
T-019027

— A plasmid based reverse genetics system for Bourbon virus was created. This system and related plasmids allow for the creation of infectious Bourbon virus and evaluate the impact of genetic variation on the virus (replication, disease, antibody-mediated neutralization etc.). It also allows for the c…

MAP3K6-knockout mouse
Boon, Adrianus "Jacco"
T-019026

— CRISPR-Cas9 gene editing was used to modify the coding region of the MAP3K6 mouse gene. The genetic modification resulted in the deletion of the full length production of the protein. This modification was made in a C57BL/6J genetic background. MAP3K6 is a signaling molecule and could be the target …

NMI knockout mice
Boon, Adrianus "Jacco"
T-019025

— CRISPR-Cas9 gene editing was used to modify the coding region of the NMI mouse gene. The genetic modification resulted in the deletion of the full length production of the protein. This modification was made the C57BL/6J genetic background. NMI is a host protein involved in innate immune signaling a…

SAMD9L-knockout mice
Boon, Adrianus "Jacco"
T-019004

— CRISPR-Cas9 gene editing was used to modify the coding region of the SAMD9L mouse gene. The genetic modification resulted in the deletion of the full length production of the protein. This modification was made in a C57BL/6 genetic background.