Summaries by Inventor Colonna, Marco
Colonna, Marco
T-019371
— Disease indication – In combination with anti-PD-1 therapy for cancer. Anti-PD-1 therapy is currently approved for: Drug format – Monoclonal antibody Drug class – First-in-class Target – TREM2 Research stage and preliminary data – The inventors performed initia…
IL-34-deficient reporter mice (IL34 LacZ/LacZ)Colonna, Marco
T-019588
— Mouse strain deficient in IL-34 production with defective Langerhans cells and microglia. These mice can be used to assess the role of the cytokine IL-34 in the development and maintenance of peripheral macrophage populations. Publication: Wang, Y., Szretter, K. J., Vermi, W., Gilfillan, S., Rossin…
CD226 (DNAM1) knockout miceColonna, Marco ; Gilfillan, Susan
T-019563
— This is a mouse knockout strain for CD26, also known as DNAM-1 (DNAX accessory molecule 1). DNAM-1 is an adhesion molecule belonging to the immunoglobulin superfamily. This mouse model was used to study the role of DNAM-1 on CD8 T cell and NK cell function in vitro and in vivo. Publication: Gilfill…
Mouse monoclonal antibodies against SARS-CoV-2 spike protein – Colonna labCella, Marina ; Colonna, Marco
T-019509
— This panel of antibodies against the SARS-CoV-2 spike protein has potential applications for research or diagnostics (e.g., sandwich ELISA to quantify or detect spikes in biological fluid).
IL34 tm1b and tm1c miceColonna, Marco ; Gilfillan, Susan ; Wang, Yaming
T-019313
— Mouse line knockouts of IL-34. IL-34 is a growth factor for macrophages, microglia, and Langerhans cells, among others. Publication: IL-34 is a tissue-restricted ligand of CSF1R required for the development of Langerhans cells and microglia
IL33 tm1b and tm1cColonna, Marco ; Gilfillan, Susan ; Yoshita, Satoru
T-019314
— The Colonna lab generated two IL-33 knockout mouse lines – a total and a conditional knockout models. IL-33 is a crucial mediator of inflammation. Publication: IL-33/regulatory T cell axis triggers the development of a tumor-promoting immune environment in chronic inflammation
IL-33 mCherry MiceColonna, Marco ; Gilfillan, Susan ; Joshita, Satoru
T-019315
— Reporter mouse line that allows for identification of cells productive IL-33 through mCherry expression. IL-33 is a molecule involved in immune system responses to helminths, allergies, and asthma. Publications:
Stimulating Innate Immune Cells for Cancer Therapy or Vaccine AdjuvantsBarrow, Alexander ; Colonna, Marco
T-016924
— Technology Description Researchers in Prof. Marco Colonna’s laboratory have identified a receptor-ligand interaction that could be harnessed to activate the innate immune system to treat cancer or enhance the response to vaccines for infectious disease. Specifically, platelet-derived growth f…
Lactobacillus reuteri deltaAratCaparon, Michael ; Cervantes-Barragan, Luisa ; Colonna, Marco ; Di Luccia, Blanda
T-018589
— Bacteria modified from the parental strain Lactobacillus reuteri 100-23 that doesn’t express the enzyme aryl aminotransferase. Culture supernatants from this bacterial strain failed to stimulate AhR reporter cells. Publication: Lactobacillus reuteri induces gut intraepithelial CD4+CD8α…
Murine Hybridoma produing IgG2b Monoclonal Antibody to Human CRTAM – Cr24.1Colonna, Marco
T-007608
— Target: Cluster of Differentation 355 protein, a.k.a. Cytotoxic and Regulatory T-Cell Molecule. Notes: Clone available is Cr 24.1. Used in flow cytometry.Monoclonal antibody to human CRTAM Monoclonal antibody (mAb) Cr24.1 was generated by immunizing BALB/c mice with CRTAM-P815 cells. Cells were injected bilaterally 4 times at weekly intervals into the hind leg. Cytosine-phosphate-guanosine (CpG) oligonucleotide was used as adjuvant (CpG 1826, 25…
Rat Hybridoma producing IgG Monoclonal Antibody to Human CD177 – Clone 4127Colonna, Marco
T-007606
— Target: Cluster of Differentation 177 protein, a.k.a. Neutrophil Specific Antigen 1. Notes: Clone available is 4127.
Rat Hybridoma producing IgG1 Monoclonal Antibodies to Mouse CD96Colonna, Marco
T-010940
— Target: Cluster of Differentiation 96 protein. Notes: Clone available is 3.3.1We generated the mAb 480.1 anti – mouse DNAM-1 (mDNAM-1) or mAb 3.1 anti – mouse CD96 (mCD96) by immunizing rats with RBL cells transfected with mDNAM-1 cDNA (RBL – mDNAM-1) or mouse CD96 cDNA (RBL-mCD96) and selecting hybridomas that reacted with 293 – mDNAM-1 or 293-mCD96 t…
Rat Hybridoma producing IgG2a Monoclonal Antibodies to Murine CD155 (TAGE4)Colonna, Marco
T-007611
— Target: Cluster of Differentiation 155 protein, a.k.a. the Polio Virus Receptor. Notes: Clone available is 4.24. Used in flow cytometry.To generate the antibodies, the researchers immunized rats with a recombinant protein consisting of the mCD155 extracellular region fused to human Fc and selected hybridomas that reacted with RMA-S–mCD155 transfectants but not with untransfected RMA-S cells. Publication: DNAM-1 promotes activ…
Rat Hybridoma producing IgG2a Monoclonal Antibodies to Murine CD226 (DNAM-1)Colonna, Marco
T-007610
— Target: Cluster of Differentiation 226 protein, a.k.a. DNAX Accessory Molecule 1. Notes: Clone available is 480.1Inventors generated the antibodies by immunizing rats with RBL cells transfected with mDNAM-1 cDNA (RBL–mDNAM-1) or mouse CD96 cDNA (RBL-mCD96) and selecting hybridomas that reacted with 293–mDNAM-1 or 293-mCD96 transfectants but not with untransfected 293 cells. Publication: DNAM-1 pro…
Rat Hybridoma producing IgG2b mAB to Mouse Natural IPC-Specific AntigenColonna, Marco
T-005806
— Target: Natural Interferon-Producing Cell-Specific Antigen Notes: Clone available is 440c
Mouse Hybridoma producing IgG3 Antibodies against Human TIGIT (WUCAM)Boles, Kent ; Colonna, Marco
T-008862
— Target: T Cell Immunoreceptor with Ig and ITIM domains, a.k.a. WUCAM Notes: Clone available is 4E1.2. Used in flow cytometry and functional studies.Mouse IgG3 was generated by immunizing BALB/c mice with WUCAMFLAG-Baf3 cells. This can be used to study T cell-dependent antibody responses. Publication: A novel molecular interaction for the adhesion of follicular CD4 T cells to follicular DC
Mouse Hybridoma producing IgG2b Monoclonal Antibodies to Human FcRL4Colonna, Marco ; Wilson, Timothy
T-007612
— Target: Fc Receptor Like 4 protein. Notes: Clone available is 413D12. Used in flow cytometry, immunoprecipitation, and functional studies.Balb/c mice were immunized three times at two week intervals with paraformaldehyde-fixed FcRL4 stable transfectants, first in CFA, then twice in IFA + CpG 1826. Three days prior to fusion with SP2/0 cells, the final immunization was performed by IP injection of irradiated cells in PBS. Hybridoma clo…
Murine Hybridoma producing IgG2b Monoclonal Antibody to Human CRTAM – Clone 134Colonna, Marco
T-007609
— Target: Cluster of Differentation 355 protein, a.k.a. Cytotoxic and Regulatory T-Cell Molecule. Notes: Clone available is 134.
Murine Hybridoma producing IgG1 Monoclonal Antibody to Human B7-H3Colonna, Marco
T-007604
— Target: Cluster of Differentiation, a.k.a. Neural Cell Adhesion Molecule. Notes: Clone name is DCN.70.
Murine Hybridoma producing IgG2b Monoclonal Antibody to Human CD85h (ILT1)Colonna, Marco
T-005807
— Target: Cluster of Differentiation 85h protein, a.k.a. Ig-Like Transcripts 1. Notes: Clone name is 24.Wistar rats were immunized with ILT1/FLAG-transfected RBL cells. ILT1 mAb was selected by flow cytometry for staining ILT1/FLAG-transfected RBL cells. This antibody can be used to study myeloid cell activation. Publication: Human Myeloid Cells Express an Activating ILT1 Receptor (ILT1) That Ass…
Murine Hybridoma producing IgG2a Monoclonal Antibody to Mouse Rae1 DeltaColonna, Marco
T-005805
— Target: Retinoic Acid Early 1 Delta protein. Notes: Clone Charlotte O1.23.
Murine Hybridoma producing IgG1 Monoclonal Antibody to Human ILT3Colonna, Marco
T-005803
— Target: Immunoglobulin transcript 3 (ILT3). Notes: Clone name is ZM4.1.To generate the hyrbidomas, 10-week-old, female BALB/c mice received an initial injection of ILT3 human IgG1 fusion protein mixed with Alu-Gel-S behind the neck. 4 weeks later, they were given a booster immunization with the same immunogen, followed after 2 weeks by a final injection of purified ILT…
Murine Hybridoma producing IgG1 Monoclonal Antibody to Human CD96Colonna, Marco
T-005414
— Target: Cluster of Differentiation 96 protein, a.k.a. T Cell-Activated Increased Late Expression protein. Notes: Clone name is MX92.39.mAbs against CD96 (clone NK92.39, mouse IgG1) was obtained by immunizing mice with NK92 cells. This antibody can be used to study how NK cells recognize tumors. Publication: Cutting Edge: CD96 (Tactile) Promotes NK Cell-Target Cell Adhesion by Interacting with the Poliovirus Receptor (CD155)
Murine Hybridoma producing IgG1 Monoclonal Antibody to Human SIRP Alpha + BetaColonna, Marco
T-005413
— Target: Cluster of Differentiation 172g protein, a.k.a. Signal Regulatory Protein Alpha and Beta. Notes: Clone name is 148.
Murine Hybridoma producing IgG1 Monoclonal Antibody to Human SIRP-B2Colonna, Marco
T-005412
— Target: Cluster of Differentiation 172g protein, a.k.a. Signal Regulatory Protein Beta-2. Notes: Clone name is LSB2.20.Monoclonal antibodies were raised in a 4-to 8 week-old female Balb/c mouse by immunozation with a recombinant GST fusion protein containing the whole extracellular domain of SIRPB1. These can be used to study T-cell activation and the induction of antigen-specific cytotoxic T-lymphocyte responses by…
Murine Hybridoma producing IgG1 Monoclonal Antibody to Human CD155Colonna, Marco
T-005410
— Target: Cluster of Differentiation 155 protein, a.k.a. Poliovirus Receptor. Notes: Clone name is SKII.4.
Murine Hybridoma producing IgG1 Monoclonal Antibody to Human CD226 (DNAM-1)Colonna, Marco
T-005408
— Target: CD226 is a glycoprotein expressed on natural killer cells, platlets, monocytes and a subset of T cells. Notes: Clone name is 11A8, used for flow cytometry.
Murine Hybridoma producing IgG1 Monoclonal Antibody to Human NKp30Colonna, Marco
T-005378
— Target: NKp30 (aka CD337) is an natural killer cell receptor. Notes: Clone name is AF29-4D12.
Murine Hybridoma producing IgG1 Monoclonal Antibody to Human CD158iColonna, Marco
T-005376
— Target: A member of the killer immunoglobulin-like receptor (KIR) family expressed on natural killer cells. Notes: Clone JJC116
Murine Hybridoma producing IgG1 Monoclonal Antibody to Human CD155 (PVR)Colonna, Marco
T-005375
— Target: CD155 is a adhesion receptor, orginally known as the cellular receptor for Polio Virus. Notes: Clone name is SK II 4.3Information on antibody against poliovirus receptor (CD155) Herein, we describe the generation of a murine antibody that recognizes poliovirus receptor (CD155). We made this antibody by immunizing mice with SK-N-S1 human neuroblastoma cells. We then validated this antibody using flow cytometry. …
Murine Hybridoma producing IgG1 Monoclonal Antibody to Human CD96 (Tactile)Colonna, Marco
T-005374
— Target: CD96 is a T cell-specific receptor indicative of T cell activation. Notes: Clone name NK92.39.1. For use in flow cytometry.Researchers generated the antibodies by immunizing mice with NK92 cells and selected hybridomas that plocked binding of PVR to NK92. These antibodies have been used to study natural killer cell target cell adhesion. Publication: Cutting Edge: CD96 (Tactile) Promotes NK Cell-Target Cell Adhesion by …
Murine Hybridoma producing Monoclonal Antibodies against Human NKp44Colonna, Marco
T-004356
— Target: NKp44 is a novel triggering surface molecular specifcially expressed by activated natural killer cells. Notes: Clone name 448.189 Marco Colonna, MDInventors generated NKp44 monocolonal antibodies by immunizing mice with a NKp44-human crystallizable fragment (Fc) fusion protein. The antibodies have been used to study innate and adaptive immune responses. Publication: Paradoxic inhibition of human natural interferon-producing cells by the activ…
Drugs to supplement microglial metabolism during treatment of Alzheimer’s diseaseColonna, Marco ; Ulland, Tyler
T-017401
— Background: Alzheimer’s disease (AD) is the 6th leading cause of death in the United States, and yet causation of the disease is poorly understood and targets for therapeutic intervention are still being identified. Recently, mutations in the immune receptor TREM2 have been found to be strongl…
Rat Hybridoma producing Monoclonal Antibodies against Mouse PDCA1Blasius, Amanda ; Cella, Marina ; Colonna, Marco
T-004377
— Target: Antibodies recognize a small leukocyte population of cells called intereron producing cells or plasmacyt-oid dendritic cells. Notes: Clone names 129c1 and 927Antibodies were produced by immunizing Wistar/CRL rats with CpG oligodeoxynucleotide 1826 or heat-killed Mycobacteria tuberculosis. The antibodies are specific for mouse IFN-producing cells (IPC). Publication: Bone Marrow Stromal Cell Antigen 2 Is a Specific Marker of Type I IFN-Producing Cells in …
Antibody blocking IL-12 and IL-23 receptors to treat inflammatory diseasesColonna, Marco ; Secca da Silva, Cristiane
T-019316
— Disease indication – Autoimmune diseases (plaque psoriasis, psoriatic arthritis, Crohn’s disease) Drug format – Monoclonal antibody Drug class – First-in-class Research stage and preliminary data – Researchers have stimulated human ILC3s with IL-23 in vitro, with and…
anti-IL-26 antibodiesCella, Marina ; Colonna, Marco
T-019424
— Mouse monoclonal antibodies recognizing human IL-26
Mouse and Human Anti-MR1 HybridomaCella, Marina ; Colonna, Marco ; Fremont, Daved ; Gilfillan, Susan ; Hansen, Ted ; Myers, Nancy
T-013681
— Target: Major Histocompatibility Complex, Class 1-Related Protein Notes: Clone available is 26.5Hybridoma clone 26.5 secreting mAb 26.5 to human and mouse MR1, an MHC-like molecule that restricts the activation of anti-microbial MIAT cells. Publication: Evidence for MR1 Antigen Presentation to Mucosal-associated Invariant T Cells
