Summaries by Inventor Qi, Zongtai

Inducible PiggyBac transposase constructs
Mitra, Robi ; Qi, Zongtai
T-019209

— Chemically inducible PiggyBac transposase constructs. The plasmids that contain mutated destabilized domains, FKBP and DHFR, were purchased from Addgene with ID 31763 and 29326, respectively. The PBase-ERT2 plasmid (mPBase-L3-ERT2) was a kind gift from Dr. Allen Bradley . The coding sequence of mPBa…

Single cell calling cards (scCC): Transposon system to simultaneously map transcription factors and quantify gene expression
Mitra, Robi ; Moudgil, Arnav ; Qi, Zongtai ; Wilkinson, Michael Nathaniel
T-018609

— Technology Description Researchers in Prof. Rob Mitra’s laboratory have developed single cell calling cards (“scCCs”), a robust and versatile self-reporting transposon system for high throughput analysis of protein-DNA binding with single cell resolution. This technology simulta…

Obligate Heterotetrameric Pairs of Cre Recombinase Mutants for Efficient Recombination at Asymmetric Sites
Corbo, Joseph ; Havranek, James ; Mitra, Robi ; Myers, Connie ; Qi, Zongtai ; Zhang, Chi
T-015523

— Background: The function of enzymes involved in homologous recombination can be exploited in the field of genetic engineering in order to generate mutations of interest. However, this machinery is not functionally efficient in post mitotic or terminally differentiated cells. Viral gene therapy techn…