Summaries by Inventor Wilson, Timothy
Mouse Hybridoma producing IgG2b Monoclonal Antibodies to Human FcRL4
Colonna, Marco ; Wilson, Timothy
T-007612
Colonna, Marco ; Wilson, Timothy
T-007612
— Target: Fc Receptor Like 4 protein. Notes: Clone available is 413D12. Used in flow cytometry, immunoprecipitation, and functional studies.Balb/c mice were immunized three times at two week intervals with paraformaldehyde-fixed FcRL4 stable transfectants, first in CFA, then twice in IFA + CpG 1826. Three days prior to fusion with SP2/0 cells, the final immunization was performed by IP injection of irradiated cells in PBS. Hybridoma clo…