pTrc-eYFP Construct

— This self-replicating plasmid is a derivative of pPMQAK1-BBa_P1010 and contains an expression-enhanced YFP gene under the control of an inducible Trc promoter. This plasmid was used to demonstrate that transgenic DNA could be introduced to Synechococcus UTEX 2973 cyanobacteria cells using conjugatio…

Toxoplasma strains

— The inventors have generated multiple gene knockout strains of T. gondii, listed below.

RH∆hx∆ku80 TIR1 (RH∆ku80∆hxgprt/pTub 5’UTR-OsTir1-3xFlag-DHFR 3’UTR-CAT):

The inventors used the CRISPR/Cas9 system for genome editing, comined with the AID degradation system to generate th…

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