Wfs1 gene knockout INS-1 cells
— Pancreatic beta cell line lacking Wfs1 gene created by CRISPR/CAS9
Wfs1 gene knockout NSC34 cells
— Neuronal cell line lacking Wfs1 gene by CRISPR/CAS9
iPSC reporter BJFF6 cell lines
— These induced pluripotent stem cell reporter cell lines were generated by introducing the following fluorescent reporters at the indicated loci:
BJFF6 cells were modified with CRISPR/Cas9 to generate these cell lines.
HFF Cell Line
— This human cell line is a generic source for culturing Toxoplasma gondii in the laboratory. It was generated by Michael White’s laboratory.
Plasmids for Cryptosporidium parvum
— These plasmids are for performing CRISPR/Cas9 gene editing in Cryptosporidium parvum
To generate a CRISPR/Cas9 plasmid for use in C. parvum, researchers used restriction cloning with SacI to insert the C. parvum U6 gene into a pUC19 vector to create pUC19-CpU6. We then inserted the C. parvum actin …
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