— These plasmids encode carboxyl ester lipase (CEL) with and without genetic variants that are associated with chronic pancreatitis.
To make the plasmids, the researchers used a full-length human CEL cDNA clone. The cDNA encoded for a 14-repeat VNTR, the variant present in the CEL MODY family. The Ec…
— Mutant zebrafish animals harboring chemically induced point mutations in the genome.
Animals: spon1b fh440, kiss1 stl82, kiss1rb fh438, pon2 fh450
— To generate a panel of antibodies against ZIKV, we serially infected Irf3/ mice 30 days apart with ZIKV MR-766 (Uganda, 1947) and ZIKV H/PF/2013 (French Polynesia, 2013). Irf3/ mice were used instead of wild-type (WT) mice, because ZIKV strains are deficient in evading type I interferon-mediated imm…
— Tangible Tool: Growth factor over-expressing cell line.
Notes: Produces conditioned media to grow various mammalian tissue stem cells.
Publication: In vitro expansion and genetic…
— Knock-in mice with a mutation in alpha-crystallin (cryaa-R49C) that causes cataracts. A knock-in plasmid was generated by cloning the 5′ and 3′ arms of the mouse RAcrystallin gene, followed by mutation and homologous recombination in mouse embryonic stem cells, which were implanted into …
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