by | Nov 20, 2019 | Colonna, Marco, Wilson, Timothy
— Target: Fc Receptor Like 4 protein.
Notes: Clone available is 413D12. Used in flow cytometry, immunoprecipitation, and functional studies.
Balb/c mice were immunized three times at two week intervals with paraformaldehyde-fixed FcRL4 stable transfectants, first in CFA, then twice in IFA + CpG 1826. Three days prior to fusion with SP2/0 cells, the final immunization was performed by IP injection of irradiated cells in PBS. Hybridoma clo…
by | Nov 14, 2019 | Colonna, Marco
— Target: Immunoglobulin transcript 3 (ILT3).
Notes: Clone name is ZM4.1.
To generate the hyrbidomas, 10-week-old, female BALB/c mice received an initial injection of ILT3 human IgG1 fusion protein mixed with Alu-Gel-S behind the neck. 4 weeks later, they were given a booster immunization with the same immunogen, followed after 2 weeks by a final injection of purified ILT…
by | Nov 14, 2019 | Colonna, Marco
— Target: Cluster of Differentiation 96 protein, a.k.a. T Cell-Activated Increased Late Expression protein.
Notes: Clone name is MX92.39.
mAbs against CD96 (clone NK92.39, mouse IgG1) was obtained by immunizing mice with NK92 cells. This antibody can be used to study how NK cells recognize tumors.
Publication: Cutting Edge: CD96 (Tactile) Promotes NK Cell-Target Cell Adhesion by Interacting with the Poliovirus Receptor (CD155)
by | Nov 13, 2019 | Austin, Stephen, Diamond, Michael, VanBlargan, Laura
— Antibodies against Western Equine Encephalitis Virus (WEEV)
Inventors demonstrated the antibodies reactivity with WEEV-infected cells or with WEEV proteins. These antibodies can be used for diagnostic and potentially therapeutic purposes.
by | Nov 13, 2019 | Colonna, Marco
— Target: Cluster of Differentiation 172g protein, a.k.a. Signal Regulatory Protein Beta-2.
Notes: Clone name is LSB2.20.
Monoclonal antibodies were raised in a 4-to 8 week-old female Balb/c mouse by immunozation with a recombinant GST fusion protein containing the whole extracellular domain of SIRPB1. These can be used to study T-cell activation and the induction of antigen-specific cytotoxic T-lymphocyte responses by…
